A simple, precise and economical Zero-order (Method A), Area Under Curve [AUC] (Method B) UV-Spectropotometric methods have been developed and validated for the estimation of Mitiglinide calcium hydrate in bulk and its formulation. The solutions of standard and sample were prepared in methanol. Mitiglinide calcium hydrate was estimated at 259 nm for the zero order UV-Spectrophotometric method, while area under the zero order spectrum of Mitiglinide calcium hydrate was measured in between 254 nm to 264 nm for AUC method. Beer’s law was obeyed in the concentration range of 100 - 600 μg / ml with r2 value 0.999 for zero order method. Similarly in AUC method, Beer’s law was obeyed in the concentration range of 100-600 μg / ml with r2 value 0.999. The precision expressed as relative standard deviation, which was within 2.0 % for the above two methods. The proposed methods were successfully applied for the determination of Mitiglinide calcium hydrate in Oral formulations. In addition, the proposed methods are simple, easy to apply, low-cost, and requires relatively inexpensive instruments.
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